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Effects of 5-aminolevulinic acid (ALA)-containing supernatants from selected Rhodopseudomonas palustris strains on rice growth under NaCl stress, with mediating effects on chlorophyll, photosynthetic electron transport and antioxidative enzymes Electron. J. Biotechnol.
Nunkaew,Tomorn; Kantachote,Duangporn; Kanzaki,Hiroshi; Nitoda,Teruhiko; Ritchie,Raymond J.
Background: Rice is globally one of the most important food crops, and NaCl stress is a key factor reducing rice yield. Amelioration of NaCl stress was assessed by determining the growth of rice seedlings treated with culture supernatants containing 5-aminolevulinic acid (ALA) secreted by strains of Rhodopseudomonas palustris (TN114 and PP803) and compared to the effects of synthetic ALA (positive control) and no ALA content (negative control). Results: The relative root growth of rice seedlings was determined under NaCl stress (50 mM NaCl), after 21 d of pretreatment. Pretreatments with 1 μM commercial ALA and 10X diluted culture supernatant of strain TN114 (2.57 μM ALA) gave significantly better growth than 10X diluted PP803 supernatant...
Tipo: Journal article Palavras-chave: 5-Aminolevulinic acid (ALA); Antioxidative enzymes; Chlorophyll fluorescence; Rhodopseudomonas palustris; Rice; Salinity.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100004
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The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields Electron. J. Biotechnol.
Nunkaew,Tomorn; Kantachote,Duangporn; Nitoda,Teruhiko; Kanzaki,Hiroshi.
The aims were to explore an appropriate isolating medium for obtaining purple nonsulfur bacteria (PNSB) for use as biofertilizers in saline paddy fields and to obtain pure cultures. We therefore chose a defined isolating medium containing 0.25% NaCl, (Glutamate-Acetate broth, GA) and a rice straw broth to compare them for numbers of PNSB obtained, time to obtain pure cultures, diversity and costs. A total of 30 water and 30 sediment samples were collected from saline paddy fields in southern Thailand and used to isolate PNSB in both the isolating media. Based on 60 samples and a period of 13 days incubation under anaerobic light conditions, a greater number of samples produced PNSB growth in GA broth after only day 3; however, after that the rice straw...
Tipo: Journal article Palavras-chave: Biofertilizer; Isolation; Paddy field; Purple nonsulfur bacteria; Rice straw broth.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600007
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